The Haskell-Luevano laboratory uses in vivo models to exam how melanocortin-specific drugs effect metabolic diseases. After a drug is created, in vitro studies are performed in order to in vivo studies help verify the safety and effectiveness of the created drug.
Melanocortin Knock-Out Mice
Feeding studies are a simple method to measure the effects of melanocortin agonist and antagonists in vivo. Mice receive intracerebroventricular (ICV) injections of a given drug and their hourly food intake is measured. An powerful melanocortin 4 receptor agonist will decrease food intake while an antagonist with increase food intake. This mouse model is necessary to test drugs that are synthesized in the lab. 4,5
The Haskell-Luevano lab has TSE metabolic chambers that can be used to examine individual mouse metabolic phenotypes such as respiratory exchange ratio (RER), food intake, water intake, ambulatory activity, running wheel activity and energy expenditure. 6,7
The Haskell-Luevano lab uses mouse running wheels to help examine how exercise influences metabolic phenotypes and gene expression in the Melanocortin pathway. 8,9
- Rowland, N. E., Schaub, J. W., Robertson, K. L., Andreasen, A., & Haskell-Luevano, C. (2010). Effect of MTII on food intake and brain c-Fos in melanocortin-3, melanocortin-4, and double MC3 and MC4 receptor knockout mice. Peptides, 31(12), 2314-2317.
- Lensing, C. J.; Doering, S. R.; Adank, D. N.; Haskell-Luevano, C. Investigating Metabolic Gender Differences with Melanocortin Antagonist SKY 2-23-7.” Proceedings of American Peptide Symposium. 2015.
- Haskell-Luevano, C., Schaub, J. W., Andreasen, A., Haskell, K. R., Moore, M. C., Koerper, L. M., . . . Xiang, Z. (2009). Voluntary exercise prevents the obese and diabetic metabolic syndrome of the melanocortin-4 receptor knockout mouse. FASEB J, 23(2), 642-655. doi: 10.1096/fj.08-109686